First, make sure you’ve thoroughly investigated the FAQ page. If your question doesn’t appear there, and it’s within Mike’s expertise to answer, he will answer it on the FAQ page near the top when he has time. (There are many areas that are outside of Mike’s wheelhouse. So he only answers questions for which he knows the answers.)
Sep 26, 2023·edited Sep 26, 2023Liked by Mike Donio
hi Mike,
Are pharmaceutical antibiotics really effective at healing a sick person? If so, then I would assume that there is some mechanism other than the reduction of bacteria that is affecting the person. I've heard that antibiotics are actually anti-inflammatory, not necessarily antibacterial. But this also would be counter-intuitive to the natural processes of healing, where an inflammatory response and a bacterial response are both utilized in these natural processes. On the other hand, if antibiotics are simply ineffective, how can millions of patients be duped into thinking that they actually work? I know a huge percentage of patients are at the tail-end of their illness and would have recovered anyways and are looking for that last ditch effort to heal so when they take antibiotics, they assume that the antibiotics are the cause of their recovery. But this can't possibly account for all cases of antibiotic healing processes, can it? You would think that antibiotics would impair the healing process, since the bacterial flora is destroyed, and of course, this is the case in terms of the long-term effects of prolonged use. Thanks.
(1) Do you know of any published valid "SARS-CoV-2" negative controls for CPE (cytopathic effect)? (excluding unpublished Stefan Lanka's experiments).
I haven't found anything in the literate thus far (pubmed, google etc). They are either omitted, invalid or undocumented.
There is this paper https://www.nature.com/articles/s41467-021-22166-4 but this for cell death not CPE ("Importantly, we saw no differences in cell number between mock and SARS-CoV-2 infected cells at the time of metabolite extraction 24 h post SARS-CoV-2 infection, suggesting no measurable difference in cell death") and the full materials and methods of the mock are not documented.
I am aware of these studies but they are not for SARS-CoV-2 and are quite dated.
ENDERS JF, PEEBLES TC. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc Soc Exp Biol Med. 1954;86(2):277-286. https://pubmed.ncbi.nlm.nih.gov/13177653/
RUSTIGIAN R, JOHNSTON P, REIHART H. Infection of monkey kidney tissue cultures with virus-like agents. Proc Soc Exp Biol Med. 1955;88(1):8-16. https://https://pubmed.ncbi.nlm.nih.gov/14357329/
COHEN SM, GORDON I, RAPP F, MACAULAY JC, BUCKLEY SM. Fluorescent antibody and complement-fixation tests of agents isolated in tissue culture from measles patients. Proc Soc Exp Biol Med. 1955;90(1):118-122. https://pubmed.ncbi.nlm.nih.gov/13273372/
BECH V, VON MAGNUS P. Studies on measles virus in monkey kidney tissue cultures. 1. Isolation of virus from 5 patients with measles. Acta Pathol Microbiol Scand. 1958;42(1):75-85. https://pubmed.ncbi.nlm.nih.gov/13508250/
Magnus, P.v., Andersen, E.K., Petersen, K.B. and Birch-Andersen, A. (1959), A POX-LIKE DISEASE IN CYNOMOLGUS MONKEYS. Acta Pathologica Microbiologica Scandinavica, 46: 156-176. https://doi.org/10.1111/j.1699-0463.1959.tb00328.x
"CPE and GFP signals were observed in Vero-E6 cells electroporated with sub-genomic RNA (sgRNA)-N alone (mock) or sgRNA-N mixed with full-length RNA transcripts (recombinant viruses) at two days after transfection. Scale bar, 100 mm."
(a) Do you know what "sub-genomic RNA (sgRNA)-N alone" means?
(b) Would you consider this as a valid negative control (i.e., no "SARS-CoV-2") for CPE?
I've read about vax aiming at reproductive system, but I can never find exact: tumor on ovaries, where grows super fast, needing ovaries, tumor removed, and not healing, with tunnels, inside ...not healing 4-5 months.
Regardless of the fact that transmission has never been proven using the scientific method, people's personal experience is that we can sometimes seem to catch colds from someone else (while other times there is no suggestion of another person). Can you explain this phenomenon?
hi Mike,
Are pharmaceutical antibiotics really effective at healing a sick person? If so, then I would assume that there is some mechanism other than the reduction of bacteria that is affecting the person. I've heard that antibiotics are actually anti-inflammatory, not necessarily antibacterial. But this also would be counter-intuitive to the natural processes of healing, where an inflammatory response and a bacterial response are both utilized in these natural processes. On the other hand, if antibiotics are simply ineffective, how can millions of patients be duped into thinking that they actually work? I know a huge percentage of patients are at the tail-end of their illness and would have recovered anyways and are looking for that last ditch effort to heal so when they take antibiotics, they assume that the antibiotics are the cause of their recovery. But this can't possibly account for all cases of antibiotic healing processes, can it? You would think that antibiotics would impair the healing process, since the bacterial flora is destroyed, and of course, this is the case in terms of the long-term effects of prolonged use. Thanks.
Hello Mike,
I have some questions.
(1) Do you know of any published valid "SARS-CoV-2" negative controls for CPE (cytopathic effect)? (excluding unpublished Stefan Lanka's experiments).
I haven't found anything in the literate thus far (pubmed, google etc). They are either omitted, invalid or undocumented.
There is this paper https://www.nature.com/articles/s41467-021-22166-4 but this for cell death not CPE ("Importantly, we saw no differences in cell number between mock and SARS-CoV-2 infected cells at the time of metabolite extraction 24 h post SARS-CoV-2 infection, suggesting no measurable difference in cell death") and the full materials and methods of the mock are not documented.
I am aware of these studies but they are not for SARS-CoV-2 and are quite dated.
ENDERS JF, PEEBLES TC. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc Soc Exp Biol Med. 1954;86(2):277-286. https://pubmed.ncbi.nlm.nih.gov/13177653/
RUSTIGIAN R, JOHNSTON P, REIHART H. Infection of monkey kidney tissue cultures with virus-like agents. Proc Soc Exp Biol Med. 1955;88(1):8-16. https://https://pubmed.ncbi.nlm.nih.gov/14357329/
COHEN SM, GORDON I, RAPP F, MACAULAY JC, BUCKLEY SM. Fluorescent antibody and complement-fixation tests of agents isolated in tissue culture from measles patients. Proc Soc Exp Biol Med. 1955;90(1):118-122. https://pubmed.ncbi.nlm.nih.gov/13273372/
BECH V, VON MAGNUS P. Studies on measles virus in monkey kidney tissue cultures. 1. Isolation of virus from 5 patients with measles. Acta Pathol Microbiol Scand. 1958;42(1):75-85. https://pubmed.ncbi.nlm.nih.gov/13508250/
Magnus, P.v., Andersen, E.K., Petersen, K.B. and Birch-Andersen, A. (1959), A POX-LIKE DISEASE IN CYNOMOLGUS MONKEYS. Acta Pathologica Microbiologica Scandinavica, 46: 156-176. https://doi.org/10.1111/j.1699-0463.1959.tb00328.x
(2) In this paper https://doi.org/10.1016/j.cell.2020.05.042 on page 431 under Figure 1(C) it states
"CPE and GFP signals were observed in Vero-E6 cells electroporated with sub-genomic RNA (sgRNA)-N alone (mock) or sgRNA-N mixed with full-length RNA transcripts (recombinant viruses) at two days after transfection. Scale bar, 100 mm."
(a) Do you know what "sub-genomic RNA (sgRNA)-N alone" means?
(b) Would you consider this as a valid negative control (i.e., no "SARS-CoV-2") for CPE?
Thank You,
Dmitry
I've read about vax aiming at reproductive system, but I can never find exact: tumor on ovaries, where grows super fast, needing ovaries, tumor removed, and not healing, with tunnels, inside ...not healing 4-5 months.
Hello Mike,
What do you think of what Augustin Sanchez has to say in this article on Single Virus Genomics found here: https://agustinsanchezcobos.substack.com/p/the-end-of-the-no-virus-people
Regardless of the fact that transmission has never been proven using the scientific method, people's personal experience is that we can sometimes seem to catch colds from someone else (while other times there is no suggestion of another person). Can you explain this phenomenon?